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by Sandra Tscherwizek
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Biological Sciences
  • Author:
    Sandra Tscherwizek
  • ISBN:
    3639109031
  • ISBN13:
    978-3639109030
  • Genre:
  • Publisher:
    VDM Verlag Dr. Müller (December 10, 2008)
  • Pages:
    80 pages
  • Subcategory:
    Biological Sciences
  • Language:
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    4.2
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Regulatory expectations include accurate identification of environmental . 16S rRNA gene clone library analysis of citrus roots revealed shifts in microbial diversity in response to pathogen infection.

We used the 16S ribosomal RNA sequence (Bacteria and archaea) as nucleotide database. The top matching (minimum 97% ) strains were used as a. suspected microorganism.

16S ribosomal RNA (or 16S rRNA) is the component of the 30S small subunit of a prokaryotic ribosome that binds to the Shine-Dalgarno sequence

16S ribosomal RNA (or 16S rRNA) is the component of the 30S small subunit of a prokaryotic ribosome that binds to the Shine-Dalgarno sequence. The genes coding for it are referred to as 16S rRNA gene and are used in reconstructing phylogenies, due to the slow rates of evolution of this region of the gene. Carl Woese and George E. Fox were two of the people who pioneered the use of 16S rRNA in phylogenetics in 1977. Multiple sequences of the 16S rRNA gene can exist within a single bacterium.

Traditional methods for microbial identification require the recognition of differences in. .DNA, Ribosomal/analysis.

Traditional methods for microbial identification require the recognition of differences in morphology, growth, enzymatic activity, and metabolism to define genera and species. Full and partial 16S rRNA gene sequencing methods have emerged as useful tools for identifying phenotypically aberrant microorganisms. We report on three bacterial blood isolates from three different College of American ed laboratories that were referred to ARUP Laboratories for definitive identification. Endocarditis, Bacterial/diagnosis.

Bacterial 16S ribosomal RNA (rRNA) genes contain nine "hypervariable regions" (V1-V9) that demonstrate considerable sequence diversity among different bacteria. Species-specific sequences within a given hypervariable region constitute useful targets for diagnostic assays and other scientific investigations. No single region can differentiate among all bacteria; therefore, systematic studies that compare the relative advantage of each region for specific diagnostic goals are needed.

Detection and identication of microorganisms by gene amplication and sequencing.

Characterization of a gene coding for 16S ribosomal RN. Gonzalez R, Hanna BA (1987) Evaluation of Gen-Probe DNA hybridization systems for the identification of Mycobacterium tuberculosis and Mycobacterium

Characterization of a gene coding for 16S ribosomal RNA. Nucl Acids Res 17: 7843–ogle Scholar. Forsman M, Sandström G, Jaurin B (1990) Identification of Francisella species and discrimination of type A and type B strains of E tularensis by 16S rRNA analysis. Gonzalez R, Hanna BA (1987) Evaluation of Gen-Probe DNA hybridization systems for the identification of Mycobacterium tuberculosis and Mycobacterium. Diagn Microbiol Infect Dis 8: 69–le Scholar. Granato PA, Franz MR (1989) Evaluation of a prototype DNA probe test for the noncultural diagnosis of gonorrhea.

Ribosomal RNA Gene Organization . The rRNA genes are highly conserved among eubacteria. The 16S ribosomal RNA gene codes for the RNA component of the 30S subunit of the bacterial ribosome. It is widely present in all bacterial species. Different bacterial species have one to multiple copies of the 16S rRNA gene.

Identification of microorganisms in positive blood cultures. Automated phenotypic identification. Samples exhibiting microbial growth were submitted to Gram staining and cultured on solid media directly from the blood culture bottles. Gram-positive cocci, Gram-positive bacilli and yeast were inoculated into the cards from colonies grown on blood agar and Gram-negative bacilli from colonies grown on MacConkey agar, all diluted in saline (. % NaCl) to a . McFarland standard. Phenotypic identification by conventional methods.

Identification of Bifidobacteria by 16S rRNA Gene Sequencing . Partial 16S rRNA gene sequences were amplified from extracted DNA using primer pair Probio Uni/Probio Rev, targeting the V3 region of the 16S rRNA gene sequence Thus, biotinylated RNA baits representative of the entire 62 nuclear genomes were produced. 3. Results and Discussion. Identification of Bifidobacteria in the Gut of Mammals.

16S ribosomal RNA (or 16S rRNA) is the component of the 30S small subunit of a prokaryotic ribosome that binds to.16S ribosomal databases.

16S ribosomal RNA (or 16S rRNA) is the component of the 30S small subunit of a prokaryotic ribosome that binds to the Shine-Dalgarno sequence. The 16S rRNA gene is used as the standard for classification and identification of microbes, because it is present in most microbes and shows proper changes.

In biopharmaceutical production areas the microbial count should be kept to a minimum to ensure the stability and cleanliness of drug products. In general, the conventional API and VITEK methods are used to characterize them. However, more and more frequently, microbiologists isolate "difficult" strains that such automated systems often fail to identify. Those "difficult" strains are often found in clean room areas, because the use of disinfectants leads to a change in the metabolic pattern and so the conventional methods provide wrong results. The author, Sandra Tscherwizek, shows an opportunity to identify such ¿difficult strains¿ via a 16S rRNA gene sequencing method based on the genetic level, and describes how to interpret the outcome. Furthermore a cost-benefit calculation was done to compare the conventional methods Vitek and API with the sequencing method. The study shows that an identification of microorganisms by 16S rRNA gene sequencing leads to higher resolution power compared to conventional biochemical methods.