- Author:Ragai R. Mitry,Robin D. Hughes
- Publisher:Humana Press; 3rd ed. 2012 edition (November 5, 2011)
- Pages:435 pages
- Subcategory:Biological Sciences
- FB2 format1938 kb
- ePUB format1346 kb
- DJVU format1613 kb
- Formats:lrf docx mobi mbr
Human Cell Culture Protocols
Human Cell Culture Protocols. Supplies techniques that can be adapted to obtain cell cultures from diseased cells to gain understanding of the pathogenic mechanisms involved. Human cell culture is not a new topic, but the development of new molecular techniques and reagents which can be used to investigate cell function and the responsible intracellular mechanisms make it a continuing requirement.
Human cell culture is not a new topic, but the development of new molecular techniques and reagents which can be used to investigate cell function and the responsible intracellular . Series: Methods in Molecular Biology volume 806. File: PDF, . 8 MB. Читать онлайн.
Human cell culture is not a new topic, but the development of new molecular techniques and reagents which can be used to investigate cell function and the responsible intracellular mechanisms make it a continuing requirement.
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and Molecular Biology. Cell Division Much more active than normal cells, cancer cells. 04 MB·18,977 Downloads. Methods in Molecular Biology™. 19 MB·23,527 Downloads·New! learning about diverse methods achievable in today's fast-paced world· Extensive online resources include. Cell Biology, Genetics, Molecular Biology, Evolution and Ecology. molecular cell biology, molecular cell biology. 06 MB·9,267 Downloads. 1995) Principles of Cell and Molecular Biology (2e) . 6.
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Human Hepatocytes: Isolation, Culture, and Quality Procedures. and their application in R&D. We here summarize the present protocol of our laboratories for cell isolation. 806:99-120 · January 2012 with 1,528 Reads. How we measure 'reads'. In addition, we discuss the necessary.
Ragai R. Mitry and Robin D. Hughes (ed., Human Cell Culture Protocols, Methods in Molecular Biology, vol. 806 .
Chemicals and Solutions The following is a list of chemicals and solutions used in tissue culture of HepG2 cells and further LMD, RNA extraction, and protein lysis. Cell Culture 1. Quickly thaw cryopreserved HepG2 cells stored in −140°C by gentle swirling of cryotube in a water bath at 37°C, taking care not to overheat the cells, . cell suspension should remain cold after thawing.
Human cell culture is not a new topic, but the development of new molecular techniques and reagents which can be used to investigate cell function and the responsible intracellular mechanisms make it a continuing requirement. This third edition of Human Cell Culture Protocols expands upon the previous editions with current, detailed protocols for the isolation and culture of a range of primary cells from human tissues. With new chapters on pancreatic cells needed for basic studies on the pathogenesis of diabetes and for their application for islet transplantation, the book also delves into protocols for hepatocytes, skin cells, lung cells, parathyroid cells, gastric cells, renal cells, adipocytes, ovarian cells, bone cells, vascular smooth muscle cells, vascular endothelial cells, regulatory T cells, blood mononuclear cells, as well as new techniques being applied to human cell culture, particularly the use of biocompatible scaffolds to grow cells, the in vitro use of laser microdissection to isolate cells from culture, and automated cell culture. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Human Cell Culture Protocols, Third Edition makes it possible for a worker with basic cell culture training, whether in the fields of cell biology, gene therapy, and cell transplantation, to prepare cell cultures of the specific cell type necessary to forward their vital research.