» » Crystallization of Nucleic Acids and Proteins: A Practical Approach (Practical Approach Series)

Download Crystallization of Nucleic Acids and Proteins: A Practical Approach (Practical Approach Series) fb2

by Arnaud Ducruix,Richard Giegé
Download Crystallization of Nucleic Acids and Proteins: A Practical Approach (Practical Approach Series) fb2
Medicine
  • Author:
    Arnaud Ducruix,Richard Giegé
  • ISBN:
    0199636788
  • ISBN13:
    978-0199636785
  • Genre:
  • Publisher:
    Oxford University Press; 2 edition (January 20, 2000)
  • Pages:
    464 pages
  • Subcategory:
    Medicine
  • Language:
  • FB2 format
    1806 kb
  • ePUB format
    1915 kb
  • DJVU format
    1989 kb
  • Rating:
    4.3
  • Votes:
    102
  • Formats:
    rtf mbr lit mobi


Arnaud Ducruix, Richard Giege. Download (pdf, 2. 4 Mb) Donate Read.

Arnaud Ducruix, Richard Giege.

Arnaud Ducruix, Richard Giegé. Crystallography is the major method of determining structures of biological macromolecules yet crystallization techniques are still regarded as difficult to perform.

Series: Practical Approach Series (Book 86). Paperback: 360 pages. ISBN-13: 978-0199632466. Product Dimensions: . x . inches. Back to top. Get to Know Us. Careers.

A Practical Approach - Practical Approach Series 86 (Paperback). to producing crystals of proteins and nucleic acids for diffraction studies. I can strongly recommend it as a standard text on crystallization that no laboratory involved in the field should be without.

Crystallization of Nucleic Acids and Proteins: A Practical Approach - Practical Approach Series 86 (Paperback). This book provides the first detailed and rational guide to producing crystals of proteins and nucleic acids for diffraction studies. Standard crystallization methods are given, along with procedures for the preparation and handling of macromolecules.

Practical Approach Series.

Crystallization of Nu. .Crystallography is the major method of determining structures of biological macromolecules, yet crystallization techniques are still regarded as difficult to perform.

A. Ducruix and R. Giege, e. Oxford University Press, 1999, 464 p. soft cover. mfr. no. Oxford University Press.

Crystallography is the major method of determining structures of biological macromolecules yet crystallization techniques are still regarded as difficult to perform.

Degradation of nucleic acids and nucleotides in several conditions with.

His major focus of study is on the identity of tRNAs and their interactions with aminoacyl-tRNA synthetases (aaRS), which are of critical importance to the fidelity of protein biosynthesis. He and his colleagues have carried out the first crystallization of a tRNA/aaRS complex, that between tRNAAsp and AspRS from yeast. Crystallization of Nucleic Acids and Proteins: A practical Approach. Arnaud Ducruix, R. Gieg. Giege and Ducruix: An introduction to the crystallogenesis of biological macromolecules Lorber and Giege: Biochemical aspects and handling of macromolecular solutions and crystals Berne, Doublie an. More). Structure of the Escherichia coli heptosyltransferase WaaC: binary complexes with ADP and heptose. Sylvestre Grizot, Michèle Salem, +6 authors Arnaud Ducruix.

Crystallography is the major method of determining structures of biological macromolecules yet crystallization techniques are still regarded as difficult to perform. This new edition of Crystallization of Nucleic Acids and Proteins: A Practical Approach continues in the vein of the first edition by providing a detailed and rational guide to producing crystals of proteins and nucleic acids of sufficient quantity and quality for diffraction studies. It has been thoroughly updated to include all the major new techniques such as the uses of molecular biology in structural biology (maximizing expression systems, sequence modifications to enable crystallization, and the introduction of anomalous scatterers); diagnostic analysis of prenucleation and nucleation by spectroscopic methods; and the two- dimensional electron crystallography of soluble proteins on planar lipid films. As well as an introduction to crystallogenesis, the other topics covered are: Handling macromolecular solutions, experimental design, seeding, proceeding from solutions to crystals Crystallization in gels Crystallization of nucleic acid complexes and membrane proteins Soaking techniques Preliminary characterization of crystals in order to tell whether they are suitable for diffraction studies. As with all Practical Approach books the protocols have been written by experienced researchers and are tried an tested methods. The underlying theory is brought together with the laboratory protocols to provide researchers with the conceptual and methodological tools necessary to exploit these powerful techniques. Crystallization of Nucleic Acids and Proteins: A Practical Approach 2e will be an invaluable manual of practical crystallization methods to researchers in molecular biology, crystallography, protein engineering, and biological chemistry.